제목 | [논문] 이상호 교수님_ Modulation of ABA Signaling by Altering VxGFL Motif of PP2Cs in Oryza sativa | ||||
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작성자 | 비임파성 장기 면역 연구센터 | 등록일 | 2017-12-12 | 조회수 | 1900 |
The abscisic acid (ABA) signaling pathway is regulated by clade A type 2C protein phosphatases (PP2CAs) in plants. In the presence of ABA, PP2Cs release stress/ABA-activated protein kinases by binding to ABA-bound receptors (PYL/RCARs) for activation. Although the wedging tryptophan in PP2Cs is critical in the interaction with PYL/RCARs in Arabidopsis and rice, it remains elusive as to how other interface regions are involved in the interaction. Here, we report the identification of a conserved region on PP2Cs, termed the VxGFL motif, which modulates the interaction with PYL/RCARs through its second and fourth residues. The effects of the second and fourth residues on the interaction of OsPP2C50 with several OsPYL/RCAR proteins were investigated by systematic mutagenesis. One OsPP2C50 mutant, VFGML (‘‘FM’’) mutant, lowered the affinity to OsPYL/RCAR3 by15-fold in comparison with the wild-type. Comparison of the crystal structures of wild-type OsPP2C50:ABA:OsPYL/RCAR3 with those composed of FM mutant revealed local conformational changes near the VxGFL motif, further supported by hydrogen-deuterium exchange mass spectrometry. In rice protoplasts,ABAsignalingwas altered by mutations in theVxGFL motif. Transgenic Arabidopsis plants overexpressing OsPP2C50 and OsPP2C50FM showed altered ABA sensitivity. Taken together, the VxGFL motif of PP2Cs appears to modulate the affinity of PP2Cs with PYL/RCARs and thus likely to alter the ABA signaling, leading to the differential sensitivity to ABA in planta.
Key words: ABA receptor, type 2C protein phosphatase, VxGFL motif, Oryza sativa |
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